MURINE STUDIES REGARDING THE VARIATION
OF OXIDATIVE STATUS IN SERUM, HEPATIC AND
BRAIN SAMPLES, AFTER ADMINISTRATION OF
SOME CNS ACTIVE DRUGS
ALINA CRENGUŢA NICOLAE1, NICULINA MITREA1, CRISTINA
MANUELA DRĂGOI1*, MARIA ZINAIDA CONSTANTINESCU1,
CRISTINA CIOFRÂNGEANU2, GHEORGHE BĂRBOI3, ANDREEA
LETIŢIA ARSENE1
1Department of Biochemistry, Faculty of Pharmacy, University of
Medicine and Pharmacy ”Carol Davila”, 6, Traian Vuia Str, sector 2,
Bucharest, Romania
2Faculty of Biology, University of Bucharest, 91-95, Splaiul
Independenţei Str, Sector 5, Bucharest, Romania
3Virology Department, Institute for Diagnosis and Animal Health, 63 Dr.
Staicovici Str, sector 5, Bucharest, Romania
*Corresponding author: manuela.dragoi@gmail.com
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Abstract:

The imbalance between the production and accumulation of reactive oxygenspecies and the body’s ability to manage them by exogenous and endogenous antioxidant defense systems leads to oxidative stress.
An important source to generate reactive oxygen species (ROS), as superoxide anions (O2•-) and hydrogen peroxide (H2O2) is the mitochondrial and microsomal system.
The present study assessed the variation of oxidative status in mice, after the intraperitoneal administration of some CNS active drugs. Serum, hepatic and brain samples were used throughout the study. A chemiluminescent method was applied in order to
evaluate the oxidative status.
Despite the fact that the brain is considered highly sensitive to oxidative damage, explained by the large amount of readily oxidizable fatty acids and poor antioxidant defenses, in our study, for cerebral and hepatic tissues we proved that the administration of tested drugs led to a decrease of the oxidative status, while in the case of serum it was developed an increase of ROS concentrations.
The oxidative stress attack limitation using therapeutic agents with additional antioxidant effect, could be a therapeutic option leading to reduced doses and adverse effects of some CNS active drugs.

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