EVALUATION OF THE POSSIBLE ENDOCRINE
DISRUPTIVE EFFECT OF BUTYLATED
HYDROXYANISOLE, BUTYLATED
HYDROXYTOLUENE AND PROPYL GALLATE
IN IMMATURE FEMALE RATS
ANCA POP1, CRISTIAN BERCE2, POMPEI BOLFA3*, ANDRAS
NAGY3, CORNEL CATOI3, ION-BOGDAN DUMITRESCU4,
LUMINITA SILAGHI-DUMITRESCU5, FELICIA LOGHIN1
1IuliuHaţieganu University of Medicine and Pharmacy, Faculty of
Pharmacy, Department of Toxicology, Cluj-Napoca, Romania
2University of Agricultural Sciences and Veterinary Medicine, Faculty of
Veterinary Medicine, Department of Animal Reproduction, Gynecology
and Obstetrics, Cluj-Napoca, Romania
3University of Agriculture Sciences and Veterinary Medicine, Faculty of
Veterinary Medicine, Department of Pathology, Cluj-Napoca, Romania
4Carol Davila University of Medicine and Pharmacy, Faculty of
Pharmacy, Department of Pharmaceutical Physics and Informatics,
Bucharest, Romania
5Babes-Bolyai University, Faculty of Chemistry and Chemical
Engineering, Department of Organic Chemistry, Cluj-Napoca, Romania
*corresponding author: pompei.bolfa@usamvcluj.ro
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Abstract:

We evaluated the possible endocrine disruptive effect of butylated
hydroxyanisole (BHA) (300mg/kg bw), butylated hydroxytoluene (BHT) (75mg/kg bw) and propyl gallate (PG) (405mg/kg bw), compounds extensively used as antioxidants in foods, food packaging, cosmetics and pharmaceuticals using the immature rat uterotrophic assay. To investigate their estrogenic and/or antiestrogenic effect we used 17-21 days old
Wistar female rats that were given suspensions of the studied compounds for three consecutive days. Absolute and relative uterus weights were significantly decreased by all three compounds, while endometrial epithelium thickness was significantly affected only by propyl gallate when compared to the negative control. The data obtained from the
present study suggests that BHA, BHT and PG indeed have endocrine disruptive effects on Wistar prepubescent female rats, the most aggressive compound being PG.

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