A NOVEL BIOSENSOR FOR ACETAMINOPHEN
DETECTION WITH ROMANIAN CLAYS AND
CONDUCTIVE POLYMERIC FILMS
ADELA MAGHEAR1, CECILIA CRISTEA1,*, ANA MARIAN2, IULIU
O. MARIAN2, ROBERT SĂNDULESCU1
1Iuliu Haţieganu University of Medicine and Pharmacy, Faculty of
Pharmacy, Analytical Chemistry Department, 4 Pasteur St, 400349 Cluj-
Napoca, Romania
2Babeş-Bolyai University, Faculty of Chemistry and Chemical
Engineering, 11 Arany Janos St, 400021, Cluj-Napoca, Romania
*corresponding author: ccristea@umfcluj.ro
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Abstract:

The development of a biosensor based on the immobilization of horseradish peroxidase (HRP) within a Romanian clay-polyetilenimine film at glassy carbon electrodes (GCEs) surfaces for acetaminophen detection is described. HRP was immobilized on the surface of the GCE with polyethyleneimine (PEI) in clay nanoporous film, a technique that
offers good entrapping and a protective environment for the biocomponent due to the hydration properties of the immobilization layer. The amperometric detection of acetaminophen was successfully achieved with a sensitivity of 6.28·10-7 M and a linear range between 5.25·10-6 M and 4.95·10-5 M.




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