DERIVATIVE UV SPECTROPHOTOMETRY
USED FOR THE ASSAY OF DIAZEPAM FROM
HUMAN BLOOD PLASMA
HADI EL LAKISS, MIHAELA ILIE*, DANIELA LUIZA BACONI,
DAN BĂLĂLĂU
”Carol Davila” University of Medicine and Pharmacy, Faculty of
Pharmacy, Toxicology Department, 6 Traian Vuia St., Bucharest,
020956, Romania
*corresponding author: m16ilie@yahoo.com
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Abstract:
Therapeutic drug monitoring is a must when dealing with benzodiazepines,
mainly at the beginning of the tratment and/or when changing the medication, in order to
avoid accidental intoxications. Unfortunately, the assay methods are not handy in smaller
medical facilities, as they require sophisticated devices, procedures and highly trained staff.
The present paper aims to develop a simple UV derivative method for the assay
of diazepam from human plasma without previous chemical separation of the drug, using
the fourth derivative of the spectra and the integrated area of the peak (valley) ranging from
306 to 333 nm. The method can be used for small diazepam concentrations (2 to 10
μg/mL), which are in the range of therapeutic blood concentrations reported for diazepam.
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