RESEARCH REGARDING THE THERAPEUTIC
VALORISATION OF THE PRODUCT ALLII CEPAE
BULBUS - CYTOTOXICITY EVALUATION
MIHAELA-ZENAIDA RISTACHE*1, VIORICA ISTUDOR1, MIHAELA
DINU2, MARIA-LIDIA POPESCU1, DIANA TUDOR1
1UMF "Carol Davila" Bucharest, Faculty of Pharmacy, Department of
Pharmacognosy, Phytochemistry, Phytotherapy
2UMF "Carol Davila" Bucharest, Faculty of Pharmacy, Department of
Botany
*corresponding author: mihaela_zenaida@yahoo.es
Download Full Article

Abstract:

The literature researches on the species Allium cepa L that placed them among the products with antimitotic properties, determined us to test the cytotoxic action of several extractive fractions (estimated by the phytobiological method Constantinescu - Triticum test) for detecting the pharmacologically active fraction.
Were assessed the hydrodistilled extract (CH), aqueous macerate (CM) and fresh juice (CS) in different concentrations, compared with colchicine (RC) and methotrexate (RM) (control sample (M) = distilled water). There were investigated: the influence on wheat caryopsis germination, root elongation and changes in mitotic film (after 24 hours – with the Nikon 2 Labophot microscope). We observed the inhibition of germination for CM, CS, RC, RM, and its stimulation in the case of CH, the inhibition of root elongation by CM, CS, RC, RM, or its stimulation by CH, and changes in the mitotic film (nucleus and nucleolus hypertrophy, placed into the cells corners, precipitated nuclear material). All these results demonstrated
the cytotoxic / cytostatic action. In conclusion, only the aqueous macerate and the fresh juice of Allium cepa have cytotoxic properties.

Top