IN VITRO EVALUATION OF BACTERICIDAL
AND ANTIBIOFILM ACTIVITY OF LONICERA
TATARICA AND VIBURNUM OPULUS PLANT
EXTRACTS ON STAPHYLOCOCCUS STRAINS
MARIANA VIORICA BUBULICA1, ION ANGHEL4*, ALEXANDRU
MIHAI GRUMEZESCU2, CRINA SAVIUC3, GEORGIANA ALINA
ANGHEL5*, MARIANA CARMEN CHIFIRIUC3, IRINA GHEORGHE3,
VERONICA LAZAR3, ALEXANDRU POPESCU1
1 Faculty of Chemistry, University of Craiova, 107 Calea Bucuresti,
200515 Craiova, Romania
2 Faculty of Applied Chemistry and Materials Science, “Politehnica”
University of Bucharest, 1–7 Polizu Street, 011061 Bucharest, Romania
3Faculty of Biology, University of Bucharest, Ale. Portocalelor 1-3,
Bucharest, Romania
4University of Medicine and Pharmacy ”Carol Davila”, Bucharest
5ENT Clinic, “Coltea” Hospital, Bucharest, Romania
*corresponding authors: ionangheldoc@yahoo.com,
dr_alina.anghel@yahoo.com
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Abstract:

The purpose of the present work was to evaluate the bactericidal/antibiofilm
efficiency of Lonicera tatarica (LT) and Viburnum opulus (VO) extracts. The LT and VO extracts were screened for their in vitro antibacterial activity against Staphylococcus aureus and S. epidermidis reference and clinical, multidrug resistant and biofilm producing strains, isolated from ear and wound infections. The quantitative assay of the antimicrobial activity
was performed by broth microdilution method in order to establish the minimal inhibitory concentration (MIC). Subsequently, the biofilm forming ability was quantified by the microtiter assay. Our results showed that the LT and VO extracts interact differently with the growth and adherence ability of S. aureus and S. epidermidis strains. LT predominantly interfered with the bacterial growth exhibiting an inhibitory effect of similar intensity
against all tested strains, while VO attenuated the ability of all tested strains to colonize the inert substratum and to form biofilms. These results are leading us to the hypothesis that Lonicera tatarica and Viburnum opulus species could be used for the development of novel antimicrobial products or strategies for fighting medical biofilms.




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