THE CYTOTOXIC POTENTIAL EFFECT OF
SOME FLAVORING AGENTS ON A MOUSE
FIBROBLAST CELL CULTURE
ALINA DUCA1*, GINA MANDA2, DANA MIHELE3
1University of Medicine and Pharmacy “Carol Davila”, Bucharest,
Faculty of Pharmacy, Traian Vuia 6, Bucharest
2INCD “Victor Babes”, Bucharest, Romania
3Clinical Hospital of Dermatovenerology “Prof. dr. Scarlat Longhin”,
Calea Serban Voda 216, Bucharest, Romania
*corresponding author: alina_duca_79@yahoo.com
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Abstract:

The study aimed to evaluate the proliferative action and cellular morphology induced by the following food flavoring agents: (±) - limonene p-methyl-1,8-diene (orange flavoring substance) and (±) -3,7-dimethyl-6-octenal (lemon flavoring substance) in cell cultures.The tests were performed on normal proliferating adherent cells from the 3T3 cell
line (mouse fibroblast) grown in DMEM culture medium (Dulbecco's Modified Eagles Medium) supplemented with 10% fetal bovine serum (SFB), glutamine and antibioticantimycotic (complete culture medium). Tests for lactate dehydrogenase (LDH) release and 3- (4,5-dimethylthiazol-2-yl) -5- (3-carboxymethoxy phenyl) -2- (4-sulfophenyl) -2 H – tetrazolium (MTS) reduction were achieved after 18 hours and 24 hours of cells incubation
in the presence of the testing substances. For testing, we considered a wide range of dilutions (1:2560000-1:2000) for lemon and orange flavoring substances, compared with the corresponding dilutions of ethanol (used as reference solvent). Orange flavoring substance reduced the number of metabolically active cells in culture at dilution 1/2000 compared to the decreased effect exerted by the scent of lemon. Lemon flavoring substance
had a moderate cytostatic effect, but the orange one was strongly cytotoxic at the dilution of 1/2000 (massive release of LDH). At dilution 1/1000 it was observed a higher cytotoxicity of both flavoring substances, especially the orange flavoring at dilution 1/4000.

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