DYNAMIC ACTIVITY OF SOME ANTIOXIDANT
ENZYMES IN PRIMARY CULTURES OF
NEURONS UNDER HYPOXIA AND ISCHEMIA
LIVIA GRATIELA MIHAI¹*, NICULINA MITREA², RALUCA IOANA
PAPACOCEA1. ANCA IOANA BADARAU¹.
¹University of Medicine and Pharmacy “Carol Davila “, Faculty of
Medicine, Physiology I Department, Bucharest, Romania.
²University of Medicine and Pharmacy “Carol Davila”, Faculty of
Pharmacy, Biochemistry Department, Bucharest, Romania.
*Corresponding author: mihaigratzy@yahoo.com
Download Full Article

Abstract:

Models of oxidative stress are important in elucidating pathologies associated with increased levels of reactive oxygen species, including stroke and neurodegenerative diseases, such as Alzheimer and Parkinson diseases. Cerebellar granule neurons primary cultures became the most popular model for the in vitro studies, bringing information about the development functionlity and pathology. In this paper, we present the results of the forme antioxidant enzymes activities (catalase, superoxide dismutase, glutathione peroxidase) under oxidative stress conditions induced by hypoxia and ischemia. The spectrophotometric methods used, allow the quantification of the production of reactive oxygen species after hypoxic incubation at 37ºC and ischemia, within one hour, two hours, and three hours by monitoring the activity of antioxidant enzymes.

Top