JURKAT E 6.1 CELL LINE STUDIES REGARDING
THE EFFECTS OF SOME BIO-INDOLS ON THE
MEMBRANE FLUIDITY
CRISTINA MANUELA DRĂGOI1, NICULINA MITREA1, ANDREEA
LETIŢIA ARSENE1*, MIHAELA ILIE2, ALINA CRENGUŢA NICOLAE1
1Department of Biochemistry, Faculty of Pharmacy, “Carol Davila”
University of Medicine and Pharmacy, Traian Vuia 6, Bucharest,
Romania
2Department of Toxicology, Faculty of Pharmacy, “Carol Davila”
University of Medicine and Pharmacy, Traian Vuia 6, Bucharest,
Romania
*corresponding author: andreeanitulescu@hotmail.com
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Abstract:

Many recent research studies have reported that membrane fluidity is highly affected by oxidative stress, namely this mechanism has been strongly correlated with the rigidization of the membrane. It has been demonstrated that chronic diseases that exhibit high oxidative stress (Alzheimer, Parkinson, diabetes, cancer) are characterized at the membrane level by an important diminishing of the membrane fluidity (rigidity). Therefore, finding medicines that increase the membrane fluidity may constitute an important therapeutic resource for oxidative stress diseases at the cellular level.
The aim of the study was the assessment of the potential modifications of the membrane fluidity induced by some bio-indols, namely melatonin, serotonin, and tryptophan.
We used an in vitro experimental model on Jurkat E 6.1 lymphoblast cell line, and we assessed the effect of the studied bio-indols on membrane fluidity, using a polarized fluorescence analysis system.

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