COLLAGEN-DOXYCYCLINE MATRICES
USED IN TISSUE ENGINEERING
IRINA TITORENCU1, MADALINA GEORGIANA ALBU2*,
LACRAMIOARA POPA3, ANTON FICAI4, LUMINIŢA ALBU2,
VICTOR JINGA1, MIHAELA VIOLETA GHICA3
1Institute of Cellular Biology and Pathology “Nicolae Simionescu”, Cell
Culture Department, 8, B.P. Hasdeu, Bucharest, Romania
2 INCDTP - Leather and Footwear Research Institute, Collagen
Department, 93, Ion Minulescu, 031215, Bucharest, Romania
3“Carol Davila” University of Medicine and Pharmacy, Faculty of
Pharmacy, Physical and Colloidal Chemistry Department, 6, Traian
Vuia, 020956, Bucharest, Romania
4University Politehnica, 313, Spl. Independentei, Bucharest, Romania
*corresponding author: albu_mada@yahoo.com
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Abstract:

Porous collagen matrices with defined physical, chemical and biological
characteristics are interesting biomaterials for tissue engineering and drug delivery systems.
In this study collagen hydrogels with the same content of doxycycline and cross-linked with different concentrations of tannic acid (0, 5, 10 and 20%) were obtained. They were rheologically characterized and dried by lyophilization in order to obtain matrices (spongious forms). The obtained matrices were analyzed by scanning electron microscopy (SEM). Their biocompatibility with endothelial cells was monitored by fluorescence and
transmission electron microscopy (TEM) and their viability was assessed by MTT assay.
The results showed that matrices allow endothelial cells to develop normally, representing promising supports both for drug delivery systems and tissue engineering.

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