VALIDATION OF A GC/HS METHOD FOR
ETHANOL QUANTITATIVE ANALYSIS USING
AS INTERNAL STANDARD TERT-BUTANOL
DIANA DORUBEŢ1,4*, CORNELIA MIRCEA2, VASILE
ASTĂRĂSTOAE3, ELENA BUTNARU1
”Gr. T. Popa” University of Medicine and Pharmacy, 16 Universităţii
Street, 700115, Iaşi, Romania
1Faculty of Pharmacy, Department of Toxicology
2Faculty of Pharmacy, Department of General and Applied Biochemistry
3Faculty of Medicine, Department of Forensic Medicine
4Vaslui County Department of Forensic Medicine, Toxicology Laboratory
*corresponding author: madfilaret@yahoo.com
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Abstract:

The aim of our study was to develop and validate a new gas chromatographic (GC) method coupled with headspace (HS) for ethanol quantitative analysis using tertbutanol as internal standard. Analysis was performed using Agilent GC 6890 N equipment with headspace G 1888. Ethanol was separated in two columns for ethanol analysis DBALC1
and DB-ALC2 with two detectors FID-1A, respectively FID-2B. The values of
retention time specifically for ethanol were 1.544 (DB-ALC1; FID-1A), respectively 1.593 minutes (DB-ALC2; FID-2B). The calibration curve was linear within the range 0.5 to 4.0 g/L, and the value of the correlation coefficient was 0.99994 (DB-ALC1; FID-1A). The accuracy and the precision of method were evaluated by analysing standard ethanol
solutions samples and by determining the recovery. The mean values of recovery ratio were between 100.12% (solution 3 g/L) and 102.22% (solution 1.5 g/L) when an ethanol/water solution was used. In forensic toxicology ethanol is usually determined from blood samples, thus we elaborated a calibration curve by using standard ethanol solution in blood.
For DB-ALC1 column and FID-1A detector the value for the detection limit was 0.067 g/L, and 0.223 g/L for the quantification limit. The method validated in our study may be be used for ethanol quantification in forensic toxicology laboratories.

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