HPTLC METHOD FOR THE SEMI
QUANTITATIVE ASSAY OF ALPRAZOLAM IN
BIOLOGICAL SAMPLES
HADI EL LAKISS, DANIELA LUIZA BACONI*, CLAUDIA MARIA
GUŢU, MIHAELA ILIE, DAN BĂLĂLĂU
”Carol Davila” University of Medicine and Pharmacy, Faculty of
Pharmacy, Toxicology Department, 6 Traian Vuia St., Bucharest,
020956, Romania
*corresponding author: daniela_baconi@yahoo.com
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Abstract:

A simple high performance thin layer chromatography (HPTLC) method for the semi quantitative determination of alprazolam in plasma and urine has been developed. Precoated glass plates (silicagel 60 F254 as stationary phase) and chloroform : methanol (90:10 v:v) as mobile phase were used. The detection was performed by densitometry in the absorbance (reflectance) mode at 254 nm. A liquid-liquid extraction procedure (with tert
buthylmethyleter) at alkaline pH has been performed. A linear regression curve with correlation coefficients over 0.99 has been obtained in the range of 0.1 μg – 2 μg/spot;
repeatability, recovery percent, detection limit (DL = 50 ng) and quantification limit (QL = 100 ng) have been demonstrated. The method can be used for alprazolam quantification at toxic/lethal levels in biological samples.




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