STUDY OF OXIDATIVE STRESS INDUCTION
AFTER EXPOSURE TO BISPHENOL A AND
METHYLPARABEN IN RATS
DANIELA-SAVETA POPA1*, BELA KISS1, LAURIAN VLASE2,
ANCA POP1, RITA IEPURE1, RAMONA PĂLTINEAN3, FELICIA
LOGHIN1
1Department of Toxicology, Faculty of Pharmacy, University of Medicine
and Pharmacy “Iuliu Haţieganu” Cluj-Napoca, 8 Victor Babeş, 400012
– Cluj-Napoca, Romania
2Department of Pharmaceutical Technology and Biopharmacy, Faculty
of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”
Cluj-Napoca, 8 Victor Babeş, 400012 – Cluj-Napoca, Romania
3Department of Pharmaceutical Botany, Faculty of Pharmacy,
University of Medicine and Pharmacy “Iuliu Haţieganu” Cluj-Napoca, 8
Victor Babeş, 400012 – Cluj-Napoca, Romania
*corresponding author: dpopa@umfcluj.ro
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Abstract:
Long exposure to small concentrations of xenoestrogens (XEs) seems to be
responsible for the increase of certain hormone-dependent diseases. The results of the research concerning the study of potentially synergic interactions between XEs are few and contradictory and need further studies. The oxidative stress is involved in the generation of several pathologies, such as cancer. We studied the oxidative stress induction in rats after exposure to bisphenol A (BPA) and/or methylparaben, two xenobiotics identified as XEs.
Malondialdehyde (MDA), a lipid peroxidation end-product, and 2,3-dihydroxybenzoic acid (2,3-DHBA), a marker of in vivo hydroxyl radical production, were used to evaluate the oxidative stress generation. The levels of MDA in rat plasma were determined by HPLCUV (detection at 307 nm) after derivatization with dinitrophenylhydrazine. 2,3-DHBA was
analyzed by LC-MS/MS in multiple reaction monitoring (MRM) mode using an ion trap mass spectrometer with electrospray negative ionization. The ion transition monitored was m/z 153 → m/z (109). The results indicated that both studied xenobiotics have the potential of oxidative stress induction in rats but simultaneous exposure does not increase the intensity of effects.
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