Development and validation of a novel RP-HPLC method for pharmacokinetic studies of gliclazide in rat
ROYA TALARI1, JALEH VARSHOSAZ2, ABOLFAZL MOSTAFAVI2,
ALI NOKHODCHI3,4*
1Food and Drug Organization, Tehran, Iran
2Pharmaceutical Research Center and Faculty of Pharmacy, Isfahan
University of Medical Sciences, Isfahan, Iran
3Medway School of Pharmacy, University of Kent, Central Ave., Chatham
ME4 4TB, Kent, UK
4Drug Applied Research Centre and Faculty of Pharmacy, Tabriz
University of Medical Sciences, Tabriz, Iran
*corresponding author: a.nokhodchi@kent.ac.uk
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Abstract:

A sensitive, simple and accurate reverse phase HPLC method was developed to determine plasma levels of gliclazide (GL) in rat. The method uses liquid-liquid extraction that is not expensive and ibuprofen as internal standard, that is easily available. Mean recovery for GL and internal standard was 80% and 82% respectively. Relative standard errors and CV% ranged from 0.47 to 8.99 and from 0.72 to 12.54% for intraday and interday HPLC injections, respectively. The limit of quantitation (LOQ) and the limit of detection (LOD) for GL in serum were 0.12 µg/mL and 0.06 µg/mL respectively. The developed HPLC method is a suitable and rapid method to be adopted for pharmacokinetic studies in rat and human.

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