HPTLC METHOD FOR THE ASSAY OF TRAMADOL AND PENTAZOCINE FROM MIXTURES
ZAINAB ABDUL JALIL EBRAHIM, DAN BĂLĂLĂU, DANIELA LUIZA BACONI*, CLAUDIA MARIA GUŢU, MIHAELA ILIE
”Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy, Toxicology Department, 6 Traian Vuia St., Bucharest, 020956, Romania
*corresponding author: daniela_baconi@yahoo.com
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Abstract:

Drug consumption highly increased in recent years and the necessity of therapeutic monitoring of certain medicines (to avoid overdosage or in detoxication treatment of addicts) resulted in the necessity to diversify the opiates analytical methods, mainly in biological matrices (body fluids, tissues, etc.)
The present paper aims to evaluate a high performance thin layer chromatography (HPTLC) method for the assay of tramadol and pentazocine from mixtures in view of further use of the method for the two analgesics assay on biological matrices (blood, urine, tissue). The two drugs were tested using silicagel 60 F254 glass plates as stationary phase and two mobile phases, consisting in cyclohexane:toluene:diethylamine (15:3:2) and acetic acid:n-buthanol:water (1:4:1). The densitometric detection was performed in the absorbance (reflectance) mode at 254 nm. The semi-quantitative analysis lead to linear regression curves with correlation coefficients over 0.99. The quantification limits, of 2.03 µg for pentazocine and 0.797 µg for tramadol, allow the quantification of the two substances at toxic levels.

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