SALMEN® – A COMPLEXATION REAGENT FOR Cr (III)
GLADIOLA ŢÂNTARU1*, CĂTĂLINA DANIELA STAN2, FLORINA
CRIVOI3
University of Medicine and Pharmacy “Gr. T. Popa”, Iaşi, Faculty of
Pharmacy, 16th Universităţii Street, Iaşi 700115, Romania
1 Department of Analytical Chemistry
2 Department of Drug Industry and Pharmaceutical Biotechnology
3 Department of Pharmaceutical Physics
*corresponding author: gtantaru2@yahoo.com
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Abstract:

The biological activity of chromium (essential oligoelement) depends on its valence and on the chemical structure of the formed complex combinations. The present spectrophotometric method has been established for the quantitative assay of Cr (III), and it is based on its complexation with N,N’–bis (salicyliden)-methylendiamine (noted Salmen®), when a stable complex is formed, which has a maximum of absorption at 326 nm. The developed method has been validated and was characterized by a good linearity in the range of 0.5–5.0 μg/mL (correlation coefficient r = 0.9999). The established detection limit (LOD) was 0.066 μg/mL and the quantification limit (LOQ) was 0.22 μg/mL. There were determined the precision of the method (RSD = 1.63%) and the accuracy (the mean recovery was established at 100.17%, the recovery values being in the range 98.0–102.3 %). The proposed and validated method was applied for the determination of Cr(III) from a nutritional supplement that contained the salt chromium picolinate, and the results were good.

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