HPLC/MS ANALYSIS OF SOLANINE IN
PHYSALIS ALKEKENGI AND SOLANUM DULCAMARA
CLAUDIA BUTNARU1*, LAURIAN VLASE2, DOINA LAZĂR1,
LUMINIŢA AGOROAEI1, MIHAI IOAN LAZĂR1
1 University of Medicine and Pharmacy „Gr. T. Popa”, Faculty of
Pharmacy, 16 University Street, 700115, Iaşi, Romania
2 University of Medicine and Pharmacy „Iuliu Haţieganu”, Faculty of
Pharmacy, 13 Emil Isac, 400023, Cluj-Napoca, Romania
*corresponding author: butclaudia@yahoo.com
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Abstract:

We developed a HPLC/MS method for the identification and determination of solanine in plants. This method was applied for samples of Solanum dulcamara (from Iaşi and Vânători Neamţ areas in Romania) and Physalis alkekengi (from Bucium and Breazu, Iaşi, Romania) during August-September 2009. Isolation of solanine was performed by extraction with a mixture consisting of water/methanol/acetic acid 49/49/2 (v/v/v) in an ultrasonic bath. Solanine was determined in concentrations ranging between 0.7963 μg/g vegetal product (leaves, Zimbru, Romania) and 4.2166 μg/g (leaves, Iaşi area) in Solanum dulcamara and between 0.092 μg/g (fruits, Bucium) and 0.2818 μg/g (stems, Bucium) in Physalis alkekengi. For Physalis alkekengi, in leaves (sample from Bucium) roots and fruits (sample from Breazu) the concentration values of solanine were below the detection limit.
The mean concentration of solanine was 21.67 times greater in samples of Solanum dulcamara than in Physalis alkekengi. For Solanum dulcamara, the highest concentration of solanine was recorded in stems.

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