A PHARMACO-TOXICOLOGICAL EVALUATION
OF BETULINIC ACID MIXED WITH
HYDROXIPROPILGAMMA CYCLODEXTRIN ON
IN VITRO AND IN VIVO MODELS
CRISTINA A. DEHELEAN1*, CODRUTA SOICA1, CAMELIA PEEV1,
SORINA CIURLEA1, STEFANA FEFLEA1, PETER KASA JR.2
1University of Medicine and Pharmacy Victor Babes Timisoara, Faculty
of Pharmacy, Eftimie Murgu Suare no.2, Romania
2University of Szeged, Department of Pharmaceutical Technology,
Hungary.
*corresponding author: cadehelean@umft.ro
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Abstract:

Betulinic acid (3β, hydroxy-lup-20(29)-en-28-oic acid, BA) is a pentacyclic
triterpene previously tested on several cell lines of neuroectodermal origin and on some in vivo tumor models, such as the melanoma model. The aim of the present work was to test its activity by increasing its water soluble fraction with gamma type cyclodextrins (hydroxipropilgamma cyclodextrin). The study consisted in a preliminary in vitro evaluation on A431 and MCF-7 cell lines and in vivo tests on fertilized eggs. The dissolution capacity in presence of cyclodextrin was analysed by the phase diagram. The main conclusion was that betulinic acid possessed an important antiangiogenic activity and a significant cytotoxicity, especially on the A431 cell line.

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