OBTAINING HIGH PURITY ANTIBODIES WITH
THERAPEUTIC POTENTIAL.
MIHAI SARANDAN1*, VALENTIN ORDODI2, IOANA SISU3,
HOREA SARANDAN1, VICTOR LORIN PURCAREA5,
MIRCEA PENESCU4,5
1Banat`s University of Agricultural Sciences, Faculty of Veterinary
Medicine Timisoara, 300645, 119 Calea Aradului, Timişoara, Romania
2University of Medicine and Pharmacy "Victor Babes" Timisoara
3Romanian Academy, Institute of Chemistry Timişoara, 300223 Mihai
Viteazul bd., no. 24, Timişoara, Romania
4“Carol Davila” Hospital of Nephrology, no. 4, Calea Grivitei str.,
010701 Bucharest, Romania
5University of Medicine and Pharmacy “Carol Davila”, no. 37, Dionisie
Lupu str., 020021 Bucharest, Romania
*corresponding author: mihai.sarandan@gmail.com
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Abstract:

The aim of this study was to provide a method for obtaining high purity egg yolk antibodies (IgY) with potential use in immunodiagnostic or for the prophylaxis and therapy of infectious diseases. The immunoglobulins were purified by extraction, repeatedprecipitation and size exclusion dialysis. The purity of the obtained antibodies was assessed by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and computerized densitometry. By our optimized method and by using dried egg powder(Globigen® Pig Doser-Ghen Corporation, Japan) as an antibody source we obtained electrophoretically pure antibodies. Results were compared with the amount of IgY extracted from egg yolk.

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