CHEMOMETRIC METHODS FOR THE
SIMULTANEOUS ASSAY OF
CHLORAMPHENICOL, CHLORHEXIDINE AND
METRONIDAZOLE DURING IN VITRO
DISSOLUTION OF DRUGS FROM
MUCOADHESIVE BUCCAL GELS.
ELENA DINTE, IOAN TOMUŢĂ*, EVA M. MUŢ, RAREŞ I.
IOVANOV, SORIN E. LEUCUŢA
Department of Pharmaceutical Technology and Biopharmaceutics,
Faculty of Pharmacy, ”Iuliu Hatieganu” University, Cluj-Napoca,
400023, Romania
*corresponding author: tomutaioan@umfcluj.ro
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Abstract:

Two chemometric methods, partial least squares regression (PLS) and principal component regression (PCR), were evaluated for the simultaneous assay of chloramphenicol, chlorhexidine and metronidazole on dissolutions samples from a mucoadhesive buccal gel formulation. A calibration set consisting of 34 mixture solutions was prepared by means of an orthogonal experimental design and their absorption spectra were recorded in the spectral range of 220–390 nm.
An external HPLC validated method was carried out by applying the developed methods to the analysis of synthetic mixtures of the related compounds, obtaining successful results. The methods were finally used to assay the studied drugs for in vitro release studies from mucoadhesive buccal gels.

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