PHENOTYPIC AND GENOTYPIC
CHARACTERIZATION OF ANTIBIOTIC
RESISTANCE PATTERNS IN ACINETOBACTER
BAUMANNII STRAINS ISOLATED IN A
ROMANIAN HOSPITAL.
MANUELA-ANDA RADU-POPESCU1*, SILVIA DUMITRIU2,
SIMONA ENACHE-SOARE3, GABRIELA BANCESCU2, AURELIAN
UDRISTOIU4, MANOLE COJOCARU5, CODRUTA VAGU6
1 University of Medicine and Pharmacy “Carol Davila, Faculty of
Pharmacy, Department of Microbiology, Traian Vuia 6, Sect. 2, 020956,
Bucharest, Romania
2 University of Medicine and Pharmacy “Carol Davila”, Faculty of
Dental Medicine, Department of Microbiology, Bucharest, Romania
3 MICROGEN- Center for Research in Microbiology, Genetics and
Biotechnology, University of Bucharest, Faculty of Biology, Bucharest,
4 Targu Jiu Hospital, Targu Jiu, Romania
5 University of Medicine and Pharmacy “Titu Maiorescu”, Faculty of
Medicine, Bucharest, Romania
6“Stefan S. Nicolau” Virology Institute, Bucharest, Romania
*corresponding author: andamrp@gmail.com
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Abstract:
A total of 633 strains of Acinetobacter baumannii were isolated from respiratory secretions (19%), blood (3.1%), urine (3.7%) and wounds secretions (4.3%). A. baumannii exhibited high resistance rates to ciprofloxacin (93.1%), amikacin (83.8%), ceftazidime (70.4%) and 21.8% were resistant to imipenem. The genotyping PCR (polymerase chain reaction) tests performed of 35 strains confirmed the presence in these MBL-producing (metallo-ß-lactamase) strains the blaIMP-1- (type carbapenemases) and blaVIM-2 (Verona integron-encoded metallo-ß-lactamase) alleles and respectively OXA-type (oxacillinase group of ß-lactamase) carbapenemases (blaOXA-24, blaOXA-51 and blaOXA-58). The
PCR amplification has highlighted this in 60% of these strains the presence of 2.2kpb amplicons characteristic for class 1 integrons.These genetic features are accounting for the epidemic potential of multiresistant clones of A. baumannii circulating in hospitals.
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