HIGH THROUGHPUT QUANTIFICATION OF QUINIDINE IN HUMAN PLASMA BY LC/MS/MS FOR THERAPEUTIC DRUG MONITORING.
LAURIAN VLASE^1*, IOANA MÎNDRUŢĂU¹, DANA MUNTEAN¹,
DANIELA IACOB², SORIN E. LEUCUŢA¹
1Faculty of Pharmacy, Department of Pharmaceutical Technology and
Biopharmaceutics,
2Faculty of Medicine, Department of Pediatrics, “Iuliu Haţieganu”
University of Medicine and Pharmacy, Cluj-Napoca, 13 Emil Isac,
400023, Romania
*corresponding author: vlaselaur@yahoo.com
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Abstract:

A simple, rapid and sensitive HPLC method for the quantification of quinidine in human plasma has been developed and validated. Quinidine was separated on a C18 column under isocratic conditions using a mobile phase of 85:15 (v/v) 0.2% formic acid and acetonitrile. In these chromatographic conditions, the retention time of quinidine was 1.2 min and the overall time of one analysis was 1.6 min. Plasma sample preparation consisted in protein precipitation with methanol. The detection of quinidine was performed in multiple reaction monitoring (MRM) mode, using an ion trap mass spectrometer with electrospray positive ionization. The linearity domain was established between 0.33 and 13.26 μg/mL. Accuracy (%) and precision (CV%) were less than 7.9% for intra-day assay and 8.9% for inter-day assay, and less than 18.7% and 22.6%, respectively, at the limit of quantification. The recovery ranged between 92.5 and 129.5%. The method is very simple and rapid and can be used for the therapeutic drug monitoring of quinidine..

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