COMPARISON OF A LC/MS METHOD WITH A LC/UV METHOD FOR THE DETERMINATION OF METFORMIN IN PLASMA SAMPLES.
CRISTINA GEORGIŢĂ1*, IULIA SORA1, FLORIN ALBU1, CRINA
MARIA MONCIU2
1 Labormed Pharma S.A. Splaiul Independentei 319, Bucharest, Romania
2 University of Medicine and Pharmacy Bucharest, Faculty of Pharmacy,
Department of Analytical Chemistry, Str. Traian Vuia 6, Bucharest,
Romania
* corresponding author: cristinageorgita@yahoo.com
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Abstract:
A LC/MS method for the analysis of the highly polar anti-diabetic drug metformin in plasma samples is compared to an ion-pair HPLC method with UV detection. Both methods showed good linearity in the concentration range of 50 to 2000 ng/mL, good precision and accuracy and similar sensitivity. The LC-MS method has the advantage of a simpler and faster preparation procedure, shorter analytical times and higher selectivity. Both methods were validated and successfully applied to bioequivalence studies of drugs containing metformin.
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