SPECTROFLUORIMETRIC METHOTREXATE
ASSAY IN HUMAN PLASMA.
MARIA BĂRCĂ*, MIHAELA ILIE, DANIELA LUIZA BACONI,
ANNE-MARIE CIOBANU, DAN BĂLĂLĂU, GEORGE T. BURCEA
”Carol Davila” University of Medicine and Pharmacy,
Faculty of Pharmacy, 6 Traian Vuia Str., Bucharest, Romania
*corresponding author: barca_monica@yahoo.com
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Abstract:
Methotrexate (MTX) is an antimetabolite used at high doses in several neoplasticdisease (acute lymphoblastic leukemia, osteosarcoma, etc.), but also, at low doses, in certain autoimmune diseases. As the treatment with MTX in neoplastic disease is always accompanied by the administration of leucovorin, acting as a “rescue” agent, the actual levels of MTX and leucovorin in patients has to be carefully monitorised.
The paper presents a simple, yet sensitive, accurate and precise spectrofluorimetric method for MTX assay in human plasma using the oxidation reaction of the drug with KMnO4 in acetate buffer. The method can be used to monitorise the MTX plasmatic level in patients treated for acute lymphoblastic leukemia or osteosarcoma.
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