DETERMINATION OF VENLAFAXINE TOXIC LEVELS FROM HUMAN
SERUM BY NON-AQUEOUS CAPILLARY ELECTROPHORESIS.
NICOLETA CARMEN PURDEL1*, DAN BĂLĂLĂU1,
MIHAELA ILIE1, CORINA CRISTINA ARAMĂ2
1Department of Toxicology, Faculty of Pharmacy, 6th Traian Vuia str,
Bucharest 2, 020956
2Department of Analytical Chemistry, Faculty of Pharmacy, 6th Traian
Vuia str, Bucharest 2, 020956
*corresponding author: carmen_purdel@yahoo.com
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Abstract:
A rapid and sensitive procedure using nonaqueous capillary electrophoresis (NACE) for the assay of venlafaxine has been developed and validated. Optimum detection of venlafaxine was obtained on a 56 cm (effective length) x 50 μm capillary, using a nonaqueous electrolyte solution system consisting of 7:3 methanol-acetonitrile with 15mM ammonium acetate, capillary temperature 250C and hydrodynamic injection, detection at 230 nm. The method is simple, accurate and reproducible and was successfully applied for the assay of toxic levels of venlafaxine in biological samples (human serum).
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