RESEARCHES REGARDING THE OBTAINING OF VEGETAL PRINCIPLES WITH POSSIBLE ANTIDOTE PROPERTIES IN LEAD POISONING.
NOTE 2. ANTIDOTE EVALUATION OF QUERCETOL IN ACUTE LEAD ACETATE POISONING ON MICE.
GHEORGHE ŢARĂLUNGĂ1,2, FLORENTINA RONCEA1*,
VIORICA ISTUDOR3
1"Ovidius" University, Faculty of Pharmacy, Constanta, Romania
2Army Center for Medical Research, Bucharest, Romania
3University of Medicine and Pharmacy "Carol Davila", Faculty of
Pharmacy, Bucharest, Romania
*corresponding author: florentinaroncea03@yahoo.com
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Abstract:
Our previous research studies on crustaceans (Euxinia maeotica) and molluscs (Mytillus galloprovincialis) acute lead acetate poisoned and treated with quercetol (3, 3’, 4’, 5,7-pentahidroxiflavone) showed antidotal and antioxidant properties for quercetol (superoxidedismutase and catalase – oxidative stress enzymes activites decreased). The present study investigated the antidotal effect of quercetol on white mice acute lead acetate poisoned. For this purpose we determined the antidotal potential of a single molar dose of quercetol (70μmoles/Kg b.w.) administered per os while lead acetate doses (toxic substance) were administered i.v., each 1 hour variated in geometric progression. The mice were divided in 6 experimental groups of 20 animals each (10 males and 10 females), and the animals were kept under observation 72 hours from the start of the treatment, until the mortality occured. We established: protection index (IP), number of toxic molecules bioinactivated by a quercetol molecule and the regression equation of the antidotal effect, depending on lead acetate doses.
The IP for a single dose of 70μmoles/Kg b.w. quercetol was 1.43, the number of toxic micromoles bioinactivated by a quercetol micromole was 2.18, the correlation coefficient (r) between the effect and the dose is 0.997, the fitting error is 0.113. The regression analysis for quercetol antidote effect varying with lead acetate dose shows that this one is considerably determined by the toxic dose variation. The small doses of quercetol used in the experiment did not influence the toxic action by its own toxicity. As a conclusion, quercetol acts as an antidote in lead acetate acute poisoning.
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