THE HPLC PLASMATIC PROFILE OF SOME FAT-SOLUBLE ANTIOXIDANT MICRONUTRIENTS (ALL-TRANS-RETINOL,
α-TOCOPHEROL, COENZIME Q10) IN DIABETIC AND DYSLIPIDEMIC PATIENTS.
VLAD GRUIA^1*, CORINA ARAMA², NICULINA MITREA¹,
ANDREEA L. ARSENE¹, DANIELA GRADINARU¹, CRISTINA DRAGOI¹
“Carol Davila”, University of Medicine and Pharmacy, Faculty of
Pharmacy, 6th Traian Vuia str, Bucharest 2, 020956
1Department of Biochemistry
2Department of Analytical Chemistry
*corresponding author: gruiavlad@yahoo.com


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Abstract:

Quantification of lipophilic vitamins in clinical or biological samples is very
important from the medical, epidemiological and informational points of view. Adequate dietary intake of nutrients such as vitamin A (retinol) and vitamin E (tocopherol) is essential for normal development and health maintenance [1].
The simultaneous analysis of vitamins A, E and coenzyme Q₁₀ are reported as a profile of fat-soluble vitamins. Decreased concentrations of these principal molecules can reveal the need for an increased dietary intake [2].
Simultaneous determination of these compounds in biological samples (plasma, serum, tissues and plant material) appears to be difficult due to their liability to photooxidation, presence of cis- and trans-isomers and their diverse polar structures [3].
In this regard, the aim of our study was to assay the plasmatic profile of the fatsoluble (lipophylic) antioxidant micronutrients (all-trans-retinol, α-tocopherol, coenzime Q₁₀) of diabetic and obese patients using a specific method for their simultaneous quantification, namely HPLC with UV detection..

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