QUALITATIVE AND SEMIQUANTITATIVE TLC ANALYSIS OF CERTAIN PHENOTHIAZINES IN HUMAN PLASMA.
LUMINIŢA BLENDEA*, DAN BĂLĂLĂU, CLAUDIA MARIA
GUŢU, MIHAELA ILIE, DANIELA LUIZA BACONI
”Carol Davila” University of Medicine and Pharmacy, Faculty of Pharmacy,
Toxicology Department, 6 Traian Vuia, 020956, Bucharest, Romania
*corresponding author: ldblendea@yahoo.com


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Abstract:

Phenothiazines have been reported to be rather often the cause of acute intoxications, either in overdoses or in associations with other medicines and / or alcohol. The paper presents a simple thin layer chromatographic method to qualitatively and semiquantitatively assay chlorpromazine, thioridazine and levomepromazine in normal human plasma spiked with the threee substances, following liquid – liquid extraction with dichloromethane. Several mobile phases were tested and methanol : n-buthanol (60:40), with 0.1 M NaBr was considered the best to be used in the determination. The analytes were found in the samples with a recovery coefficient of 42%, 78% and 67% for chlorpromazine, thioridazine and levomepromazine, respectively..




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