EXPERIMENTAL ASSESSMENT OF CEREBRAL
MONOAMINERGIC STATUS IN A MURINE
MODEL OF BEHAVIOR.
ANDREEA LETIŢIA ARSENE1, CORINA ARAMĂ2, NICULINA
MITREA1, AURELIA NICOLETA CRISTEA3
1Department of Biochemistry, University of Medicine and Pharmacy
“Carol Davila”, Faculty of Pharmacy, Bucharest, Romania
2Department of Analytical Chemistry, University of Medicine and
Pharmacy “Carol Davila”, Faculty of Pharmacy, Bucharest, Romania
3Department of Pharmacology, University of Medicine and Pharmacy
“Carol Davila”, Faculty of Pharmacy, Bucharest, Romania
*corresponding author: andreeanitulescu@hotmail.com
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Abstract:
The purpose of our study was to evaluate some biochemical markers of neuronal
metabolism in a classical pattern of behavior, developed by Friedman and Rosenman
(1974), the adrenergic “A” type. Epidemiologic studies demonstrated an association
between type A behavioral characteristics and specific physipathologic conditions:
hypersensitivity to pain, increased heart rate, high systolic blood pressure, bipolar disorders,
or specific preferences for atherogenic diet, despite the fact that molecular basis of these
findings have not been established, yet.
Mice were divided in two behavioral groups, according to their reactivity to
painful stimulus (endogenous analgesia): type A (associated with hypersensitivity to pain)
and type B (“non-A”, the opposite type of behavior) which exhibits hypo reactivity to pain.
Mice pain sensitivity was assessed using the hot-plate test (60ºC).
Cerebral monoaminergic status was assessed by measuring the levels of
noradrenaline (NA), dopamine (DA), serotonin (5-HT) using HPLC with electrochemical
detection, and γ-aminobutiric acid (GABA) using a fluorimetric assay.
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