ANTIMICROBIAL ACTIVITY OF SOME NEW
THIOUREIDES FROM 2-THIOPHENEACETIC ACID.
CARMELLINA DANIELA BĂDICEANU1*, CRISTINA LARION2
1Pharmaceutical Chemistry Department, Faculty of Farmacy, „Carol
Davila” University of Medicine and Pharmacy , Traian Vuia 6, sect. 2,
020956, Bucharest, Romania
2Microbiology-Immunology Department, Faculty of Biology, University
of Bucharest, Ale. Portocalelor 1-3, sect. 5, Bucharest, Romania
* corresponding author: bcarmellina@yahoo.com
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Abstract:

The aim of this study was to evaluate the „in vitro” antimicrobial activity of nine new compounds derived from 2-thiopheneacetic acid using qualitative and quantitative methods. These compounds have been synthesized and characterized in a previous paper [7].
The qualitative screening of the susceptibility spectra of different microbial
strains to these compounds was performed by three adapted diffusion methods: paper filter disk impregnation with the tested substances solutions in DMSO (dimethyl sulfoxide), the disposal of the tested solutions in agar wells and the spotting of the tested solutions on solid medium seeded with microbial inoculums. The quantitative assay of the antimicrobial activity was performed by broth microdilution method in 96-well microplates in order to establish the minimal inhibitory concentration (MIC).The antimicrobial activity was tested against Gram-positive strains (Staphylococcus (S.) aureus, Bacillus (B.) subtilis), Gramnegative (Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, Klebsiella (K.) pneumoniae) and fungal strains (Candida (C.) albicans).
As concerning the Gram positive strains, the tested compounds exhibited no significant antimicrobial activity against S. aureus, but two of these compounds were active on B. subtilis. All tested compounds exhibited antifungal activity against C. albicans and four of them against other Gram-negative clinical strains, especially K. pneumoniae.

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