ION-PAIR REVERSED-PHASE HIGHPERFORMANCE
LIQUID CHROMATOGRAPHY
OF ONDANSETRON HYDROCHLORIDE USING
SODIUM HEPTANESULPHONATE AS A
COUNTERION.
ANDREEA VARVARA1*, CRINA-MARIA MONCIU2,
CORINA ARAMĂ2, C. POPESCU1
1Raw Materials and Finished Products Control Department, National
Medicines Agency, Av. Sanatescu Street no. 48, Bucharest, Romania
2Analytical Chemistry Department, Faculty of Pharmacy, UMF Carol
Davila, Traian Vuia Street no. 6, Bucharest, Romania
*corresponding author: andreea.varvara@anm.ro
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Abstract:

A simple and selective isocratic HPLC method for the determination of ondansetron hydrochloride has been developed. The separation is achieved by ion-pair reversed-phase chromatography on a Ultrasphere C8 column. A mixture of 0.3% sodium heptanesulphonate in phosphate buffer solution pH 3.0 and methanol (40/60, V/V) is used as mobile phase. The influence of pH, mobile phase composition and concentration of organic modifier on capacity factor of ondansetron has been investigated. The HPLC system is applied to the quantitative estimation of the active substance in bulk and pharmaceutical dosage forms.




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