THE EVALUATION OF ANTIOXIDANT
POTENTIAL OF VERONICA OFFICINALIS AND
ROSMARINUS OFFICINALIS EXTRACTS BY
MONITORING MALONDIALDEHIDE AND
GLUTATHIONE LEVELS IN RATS.
BÉLA KISS1,*, DANIELA-SAVETA POPA1, GIANINA CRIŞAN2,
MARIUS BOJIŢĂ3, FELICIA LOGHIN1
1Department of Toxicology, University of Medicine and Pharmacy “Iuliu
Haţieganu”, Faculty of Pharmacy, no. 6 Pasteur, 400349, Cluj-Napoca,
Romania
2Department of Pharmaceutical Botany, University of Medicine and
Pharmacy “Iuliu Hatieganu”, Faculty of Pharmacy, no. 13 Emil Isac,
400023, Cluj-Napoca, Romania
3Department of Drug Analysis, University of Medicine and Pharmacy
“Iuliu Haţieganu”, Faculty of Pharmacy, no. 6 Pasteur, 400349, Cluj-
Napoca, Romania
*corresponding author: kbela@umfcluj.ro
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Abstract:

Malondialdehide and glutathione are two of the main biomarkers of oxidative stress. These biomarkers were used to evaluate the antioxidant potential of two plant extracts, obtained from Rosmarinus officinalis and Veronica officinalis, in Wistar rats. In the case of malondialdehide the assay was based on derivatization with dinitrophenylhydrazine, followed by chromatographic analysis with UV detection at 307nm. The reduced and total glutathione were quantified from rat plasma, after derivatization with o-phtalaldehyde, using a HPLC method with fluorescence detection. The results indicated a potential protective effect of these plants against oxidative stress.

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