PHARMACOGNOSTIC RESEARCH ON VIOLA
DECLINATA WALDST. ET KIT. (VIOLACEAE).
ANCA TOIU1, EDWARD MUNTEAN2, ILIOARA ONIGA1,
MIRCEA TĂMAŞ1
1Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu
Haţieganu” Cluj-Napoca, Romania
2University of Agricultural Sciences and Veterinary Medicine Cluj-
Napoca, Romania
*corresponding author: ancamaria_toiu@yahoo.com
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Abstract:
In order to continue our previous studies on some Viola species from Romania,
we have analysed for the first time some active principles from aerial parts of V. declinata
Waldst. et Kit. We have performed gravimetric methods for saponins and mucilages, and
spectrophotometric determinations and HPLC analysis of carotenoids. We established the
content in saponins (4.40%), mucilages (10.26%), and total carotenoids (8.45 mg/100g
vegetal product, expressed in β-carotene). In fresh aerial parts we have determined by
HPLC: violaxanthin (281 μg/100g), antheraxanthin (283 μg/100g), lutein (896 μg/100g),
zeaxanthin (479 μg/100g), β-carotene 5,6-epoxide (48 μg/100g), β-carotene (546 μg/100g),
and 9Z-β-carotene (76 μg/100g). Our results showed the presence of the same active
principles in Viola declinata and V. tricolor, the differences being only quantitative.
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