SEPARATION OF CO-EXISTING PARACETAMOL AND DICLOFENAC SODIUM ON SILICA GEL ‘H’ LAYERS USING SURFACTANT MEDIATED MOBILE PHASES: IDENTIFICATION OF DICLOFENAC SODIUM FROM HUMAN URINE.
ALI MOHAMMAD*, SUDHANSHU SHARMA
Analytical Research Laboratory, Department of Applied Chemistry,
Faculty of Engineering & Technology, Aligarh Muslim University,
Aligarh-202002 INDIA
*corresponding author: alimohammad08@gmail.com
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Abstract:

Thin layer chromatography (TLC) has been used as an analytical technique for the identification and separation of two different drugs (paracetamol and diclofenac sodium) on silica gel H layers under optimized experimental conditions using surfactant mediated mobile phase systems. Cationic, non-ionic and anionic surfactants at different concentration levels
have been used as mobile phases. Among the TLC systems studied, silica gel H used as stationary phase and methanol + 1% aqueous N-cetyl-N,N,N-trimethylammonium bromide (CTAB) + acetic acid (4:5:1 v/v/v) used as mobile phase were most favorable for achieving mutual separation of paracetamol and diclofenac sodium. The effect of forced degradation parameters such as sunlight, pH variation and storage temperature on the mobility (R_F) of drugs have been studied using 1% aqueous N-cetyl-N,N,N-trimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) as eluents. This chromatographic system [silica gel H (stationary phase) and methanol + 1% aqueous CTAB+ glacial acetic acid (mobile phase)] is also used in order to identify diclofenac sodium from human urine..

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