DETERMINATION OF SOME ISOFLAVONOIDS AND FLAVONOIDS FROM GENISTA TINCTORIA L BY HPLC-UV.
AMELIA TERO-VESCAN*, SILVIA IMRE, CAMIL EUGEN VARI, ALEXANDRINA OŞAN, MARIA T.DOGARU, CAROL CSEDÖ
University of Medicine and Pharmacy Tg-Mures, Faculty of Pharmacy, 540139 Tg-Mures, Romania
*corresponding author: terovescan@yahoo.com
Download Full Article

Abstract:

Genista tinctoria L, Fabaceae family is a wide spread plant in Romania, from the plain region to the mountains. Despite this, it is not studied very well. Few bibliographic data prove the presence of flavonoidic and isoflavonoidic compounds that have phytoestrogenic effects out of which the most important are: genistein (GNST) and daidzein (DDZ). The purpose of this study was to obtain a simple and precise method to isolate, purify and quantify by a HPLC method, some of the most important flavonoidic and isoflavonoidic compounds in Genista tinctoria L. Separation and quantification of flavonoidic and isoflavonoidic compounds was performed by high performance liquid chromatography using a gradient method on a reversed phase C18 column.

Top