QUANTITATIVE ANALYSIS OF BIO- ACTIVE COMPOUND IN HIBISCUS SABDARIFFA L. EXTRACTS. NOTE I
QUANTITATIVE ANALYSIS OF FLAVONOIDS.
SUHAD S. HUMADI, VIORICA ISTUDOR
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Abstract:

In the phytochemical extraction researches there has been an increasing demand to find a simple, rapid, and precise method for the quantitative analysis of flavonoids (flavones, anthocyanins, and tannins) in plant extracts. This paper describes the quantitative determination of flavonoids (flavonols) calculated as quercetin equivalent in different Hibiscus sabdariffa extracts analyzed using different spectrophotometric methods: Christ-Müllers, Chang et al and some modified methods, to obtain extracts with the highest flavonoid content showing hypoglycemic (proved by experiments performed on aloxan induced diabetic rats) and anti-oxidant activities. The obtained results indicated that the flavonoid percent determined using the Christ-Muller's method showed the lowest value among all other methods, while the highest percentual value was analyzed via Chang et al method used for the determination of both the free aglycone obtained by hydrolysis with 4 N HCl for 30 minutes and glycosylated form of flavonols.

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