SCREENING OF THE ANTIMICROBIAL ACTIVITY OF SOME NEW DIBENZO[b,e]THIEPINE DERIVATIVES. NOTE 1.
CAMELIA ELENA STECOZA, CARMEN BALOTESCU CHIFIRIUC, ANCA MICHAELA ISRAIL
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Abstract:

The emergence of the antimicrobials resistance and multiresistance of bacterial and fungal infectious agents has urged the research for new antimicrobial substances and for new strategies for the treatment of infectious diseases which still remain a top public health problem in the entire world. The aim of this study was to evaluate the in vitro antimicrobial activity of some newly synthesized compounds, O-acyloximino-dibenzo[b,e]thiepins (series 1) and O-acyloximino-dibenzo[b,e]thiepin-5,5-dioxides (series 2). The qualitative screening of the susceptibility spectra of different microbial strains to these compounds was performed by three adapted diffusion methods: paper filter disk impregnation with the tested substances solutions, the disposal of tested solutions in agar wells and the spotting of tested solutions on solid medium seeded with microbial inoculums. The in vitro antimicrobial testing was performed by binary microdilution method, in 96 multi-well plates, in order to establish the minimal inhibitory concentration (MIC), against Gram-positive: Staphylococcus aureus, Bacillus sp., Listeria monocytogenes, Gram-negative Escherichia coli, Salmonella sp., Pseudomonas aeruginosa bacteria and Candida strains. The first series of compounds exhibited different levels of antimicrobial activity, being specifically active on Pseudomonas aeruginosa and Candida albicans, while the new sulfones (series 2) exhibited low MIC values on Escherichia coli strains.
Our studies demonstrated that among other biological activities of these compounds, some of them also exhibit selective and effective antimicrobial properties that could lead to the selection and use of these compounds as efficient antimicrobial agents, especially for the treatment of multidrug resistant infections.

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