HPLC ANALYSIS OF FUROSEMIDE IN RAT PLASMA. BIOAVAILABILITY STUDY. NOTE 1.
CODRUŢA ŞOICA, SILVIA IMRE, C. VARI, Á. GYÉRESI, CRISTINA DEHELEAN, MARIA DOGARU
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Abstract:

Furosemide is a benzoic acid derivative with a powerful diuretic activity used in the treatment of edemas and hypertension. Cyclodextrins are toroidal shape oligosaccharides with a cavity that can accommodate a large number of pharmaceuticals.
In this, article a HPLC method for the separation and identification of furosemide in rat plasma is presented in order to perform a bioavailability study concerning inclusion complexes of furosemide and randomly methylated β-cyclodextrin (RAMEB). Under the mentioned HPLC parameters, the separation of furosemide (FS) and internal standard (ISTD) was specific to endogenous compounds, without significant interferences in analytes retention times from endogenous compounds. The method proved to be linear between 0.020 – 10.00 μg/mL FS and the calibration model was accepted.

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