BIOCHEMICHAL EFFECTS OF SOME NEW MANNICH BASE FROM ORTHO-HYDROXYARYL ALKYL KETONES ON RATS WITH CHRONIC INFLAMMATION.
IRINA POPOVICI, IULIANA POPOVICI, AFRODITA DOINA MĂRCULESCU
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Abstract:

The aim of our research was to determine the lethal dose 50% (LD₅₀) of an original substance, Mannich Base. The effects of this substance in chronic inflammation and on the immunity system of the experimental animals were determined. A series of Mannich bases hydrochlorides was prepared by the reaction of 2-hydroxy-5-methyl-acetophenone with paraformaldehyde and some secondary amine hydrochlorides, in 2-propanol. Some characteristics of ¹H-NMR and and ¹³C-NMR spectra, which are agreement with structure of the synthesized compounds, are described. The NBT (Nitro Blue Tetrazolium) test, serum complement and biochemical parameters for the erythrocytes of rats with experimental chronic inflammation, treated with Mannich base in dose 1/5 and 1/10 of LD₅₀ were measured. The animals for experiment were white rats, which received sterile cotton pellets, thus creating a chronic inflammation process. Our research on the proinflammatory effects of the substance showed their immunomodulatory capacity according to the biochemical effects caused by the oxidative stress.

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