THE EFFECTS OF HEMODIALYSIS UPON THE CONCENTRATION OF HOMOCYSTEINE AND REACTIVE OXYGEN SPECIES .
ANGELA ANTONESCU, MARIANA MUREŞAN, OTILIA MICLE, LIANA MICLE, LUCIANA DOBJANSCHI, CAMELIA MRAZ, M. DOROFTEIU
Download Full Article

Abstract:

Numerous recent studies have revealed a high incidence of hyperhomocysteinemia in renal patients. The aim of this paper was to point out the associations between the level of homocysteine and the oxidative stress in patients submitted to hemodialysis. We investigated 30 chronic patients during 5 months from May till September 2007, before and after the periodic hemodialysis. In order to prove the oxidative stress, we evaluated the level of serum malondyaldehide (MDA) using the tiobarbituric acid method, the concentration of carbonylated proteins being determined in the presence of guanidine hydrochloride and the level of circulating ceruloplasmin measured with the Ravin method. The concentration of homocysteine was determined using an enzymatic method. We also measured a few biochemical parameters such as urea, uric acid, creatinine, total proteins. In the control group we made the same investigations. The results pointed out an increased level of homocysteine before dialysis, also a high concentration of MDA and carbonylated proteins and a low level of ceruloplasmin. After hemodialysis, the MDA and carbonylated proteins concentration are almost the same. The antioxidant response of the organism was absent, which was also demonstrated by the unchanged level of ceruloplasmin. After hemodialysis, the level of homocysteine was lower. Regarding the biochemical parameters, urea and creatinine presented high level but the rest of them were in normal range. Increased plasma concentration of homocysteine and oxidative stress markers are found in patients submitted to hemodialysis.

Top