EXPERIMENTAL RESEARCH REGARDING MELATONIN’S EFFECTS UPON THE ADRENERGIC INNERVATION OF THE MOUSE ISOLATED VAS DEFERENS.
ANDREEA LETITIA ARSENE, NICULINA MITREA, CRISTINA DRAGOI
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Abstract:

Melatonin’s pharmacologic and physiopathologic potential constitutes the object of modern research regarding organisms’ integration mechanisms within the great informational variety of the environment. Nowadays melatonin’s effects are considered both for the central neural structures and especially for the peripheric ones. The present study aims at investigating melatonin’s effects upon the adrenergic innervation of the vas deferens isolated from mouse. The experiments were performed in vitro, using the organ bath, after incubating the isolated vas deferens with the physiological dose of melatonin. In this respect, we determined the neurogenic contractile response of the mouse vas deferens using the following conditions: electrical stimulation (trains of 5, 50 and 100 pulses of 0.5ms duration and a frequency of 10 Hz), neurotransmitter stimulation (noradrenaline 100µM) and α1-adrenergic stimulation (phenylephrine 10µM and 100 µM). The analysis criteria for all experimental data was based upon the maximum amplitude of the vas deferens’ biphasic contractions:”twitch” (purinergic, fast) and “hump”(noradrenergic, slow). We also evaluated the contraction time variation for the vas deferens considered. The experimental results evoke the bound between melatonin and the neurogenic adrenergic component of contraction of the mouse vas deferens. In this respect, the pineal hormone reduces, statistically significant, both the vas deferens contraction force and also the time required for noradrenaline to be released from vesicles.

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