METHADONE ISOLATION FROM HUMAN PLASMA BY LIQUID-LIQUID EXTRACTION AND SOLID-PHASE EXTRACTION. COMPARATIVE STUDY.
DANIELA-SAVETA POPA, BELA KISS, LAURIAN VLASE, DANA MUNTEAN, FELICIA LOGHIN
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Abstract:

Optimal experimental conditions for the methadone isolation from human plasma were established for ulterior quantification of the drug by performant analytical methods. The methadone isolation efficiency was determined by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) depending on the pH. Different organic solvents (ethyl ether, ethyl acetate and hexane) or solvent mixtures and different SPE cartridges were tested. The methadone was quantified by an original LC-MS-MS method. Maximum recovery for the extraction was obtained with solvents as ethyl ether (recovery 99.05%, n = 3) or hexane (recovery 100.75%, n = 3) without pH adjustment, and with Sep C18 cartridges (recovery 98.24%, n = 3) at pH 6.85. LLE showed the same efficiency as SPE for the methadone isolation from plasma, depending on the experimental conditions: solvent or solid phase nature, and the pH of the extraction medium. The best extraction methods can successfully be applied in clinical and forensic toxicology for the efficient isolation of methadone from human plasma samples.

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