REACTIVE OXYGEN SPECIES GENERATION IN THE APOPTOTIC RESPONSE TO OXALIPLATIN OR CISPLATIN IN HUMAN
HCT116 p53wt COLON CANCER CELLS.
CLAUDIA BURZ1, MARIA C.SHOSHAN2, STIG LINDER2, MARIA BERNDTSSON2, S.E.LEUCUŢA1
1 University of Medicine and Pharmacy “Iuliu Haţieganu” Cluj- Napoca, Romania, 13 Emil Isac
2Cancer Center Karolinska, Department of Oncology-Pathology, Karolinska, Institute And Hospital, S-17176, Stockholm, Sweden

Abstract:

The aim of this study was to determine the apoptosis induced by Oxaliplatin and Cisplatin depending of reactive oxygen species (ROS) generation. We quantified the level of apoptosis induced over 24 h by Oxaliplatin and Cisplatin, using an apoptosis-specific assay (Apoptosense TM, PEVIVA AB, Stockholm, Sweden). Total ROS levels were assessed by spectrophotometry and flow-cytometry.
The results show that Oxaliplatin and Cisplatin induced apoptosis on the HCT116 p53wt cells lines in the concentration dependent manner and the apoptosis was partially prevented by the cell-permeable peroxynitrite scavenger PTIO. In addition the induction of ROS during apoptosis occurred as early as 1-2 h post-treatment.

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